Review



il2rb mut mice young cohort t cell analysis  (Mendeley Ltd)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Mendeley Ltd il2rb mut mice young cohort t cell analysis
    Genotypes shown include WT (black) and <t>Il2rb</t> Mut (red). (A)–(E) each represent three individual experiments. (A) Total body or spleen weight of 6- to 8-week littermates, n = 15 per genotype. (B) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) comparing mice at age 4 weeks (left, WT, n = 5; Il2rb Mut , n = 6) and 7 weeks (right, IL-2, n = 19 per genotype; IL-15c, n = 17 for WT and n = 18 for Il2rb Mut ). (C) Representative dot plot depicting Tregs defined as CD4 + CD25 + Foxp3 + for genotypes indicated (left). Percentage of Tregs from CD4 + T cells, n = 11 per genotype (right). (D) Representative dot plot depicting subpopulations of CD4 + or CD8 + T cells: naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for genotypes indicated (left). Stacked graphs showing T cell distribution, n = 11 per genotype (right). (E) Absolute numbers of Tregs–CD4 + CD25 + Foxp3 + (left), n = 11 per genotype. Absolute numbers of TEM–CD44 + CD62L − cells (right), n = 7 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor; displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns, non-significant.
    Il2rb Mut Mice Young Cohort T Cell Analysis, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il2rb mut mice young cohort t cell analysis/product/Mendeley Ltd
    Average 86 stars, based on 1 article reviews
    il2rb mut mice young cohort t cell analysis - by Bioz Stars, 2026-06
    86/100 stars

    Images

    1) Product Images from "A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency"

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.115902

    Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(E) each represent three individual experiments. (A) Total body or spleen weight of 6- to 8-week littermates, n = 15 per genotype. (B) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) comparing mice at age 4 weeks (left, WT, n = 5; Il2rb Mut , n = 6) and 7 weeks (right, IL-2, n = 19 per genotype; IL-15c, n = 17 for WT and n = 18 for Il2rb Mut ). (C) Representative dot plot depicting Tregs defined as CD4 + CD25 + Foxp3 + for genotypes indicated (left). Percentage of Tregs from CD4 + T cells, n = 11 per genotype (right). (D) Representative dot plot depicting subpopulations of CD4 + or CD8 + T cells: naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for genotypes indicated (left). Stacked graphs showing T cell distribution, n = 11 per genotype (right). (E) Absolute numbers of Tregs–CD4 + CD25 + Foxp3 + (left), n = 11 per genotype. Absolute numbers of TEM–CD44 + CD62L − cells (right), n = 7 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor; displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns, non-significant.
    Figure Legend Snippet: Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(E) each represent three individual experiments. (A) Total body or spleen weight of 6- to 8-week littermates, n = 15 per genotype. (B) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) comparing mice at age 4 weeks (left, WT, n = 5; Il2rb Mut , n = 6) and 7 weeks (right, IL-2, n = 19 per genotype; IL-15c, n = 17 for WT and n = 18 for Il2rb Mut ). (C) Representative dot plot depicting Tregs defined as CD4 + CD25 + Foxp3 + for genotypes indicated (left). Percentage of Tregs from CD4 + T cells, n = 11 per genotype (right). (D) Representative dot plot depicting subpopulations of CD4 + or CD8 + T cells: naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for genotypes indicated (left). Stacked graphs showing T cell distribution, n = 11 per genotype (right). (E) Absolute numbers of Tregs–CD4 + CD25 + Foxp3 + (left), n = 11 per genotype. Absolute numbers of TEM–CD44 + CD62L − cells (right), n = 7 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor; displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns, non-significant.

    Techniques Used: MANN-WHITNEY

    Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(H) each represent 2 individual experiments. (A) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT, n = 14 for Il2rb Mut ; isotype control antibody MFI is indicated by the gray dotted line. (B) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (top) and Il2rb Mut (bottom). (C) ImageStream quantification, with membrane intensity/area. (D) CD25 expression depicted as in (A), n = 8 for WT and n = 9 for Il2rb Mut . (E) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 7 per genotype. (F and G) Activation and exhaustion markers PD-1 (F) and KLRG1 (G) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT and n = 14 for Il2rb Mut . (H) Representative dot plot distinguishing BM subsets, specifically LSK (Sca-1 + c-Kit + ) cells (top right quad) and myeloid progenitors (MPs; top left quad). Graphs show percentage of LSK cells (left) and MP cells (right), n = 6 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.
    Figure Legend Snippet: Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(H) each represent 2 individual experiments. (A) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT, n = 14 for Il2rb Mut ; isotype control antibody MFI is indicated by the gray dotted line. (B) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (top) and Il2rb Mut (bottom). (C) ImageStream quantification, with membrane intensity/area. (D) CD25 expression depicted as in (A), n = 8 for WT and n = 9 for Il2rb Mut . (E) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 7 per genotype. (F and G) Activation and exhaustion markers PD-1 (F) and KLRG1 (G) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT and n = 14 for Il2rb Mut . (H) Representative dot plot distinguishing BM subsets, specifically LSK (Sca-1 + c-Kit + ) cells (top right quad) and myeloid progenitors (MPs; top left quad). Graphs show percentage of LSK cells (left) and MP cells (right), n = 6 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Techniques Used: Expressing, Fluorescence, Control, Membrane, Staining, Activation Assay, MANN-WHITNEY

    Mixed (WT:WT or Mut:WT at 97:3, into WT) bone marrow chimera (BMC) mouse cells and sera were harvested and analyzed 12–14 weeks post-transplant. Black open shapes indicate WT cells from Mut:WT BMCs (WT/Mut:WT). Red open shapes indicate Il2rb Mut cells from Mut:WT BMCs (Mut/Mut:WT). Data depict one representative experiment in each of (A)–(I). (A) Serum cytokine levels of IL-2, n = 3 for WT:WT and n = 4 for Mut:WT, and of IL-15c, n = 2 for WT:WT and n = 4 for Mut:WT, measured as in . (B) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 6 per genotype; isotype control antibody MFI is indicated by the gray dotted line. (C) CD25 expression depicted as in (B), n = 6 per genotype. (D) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (CD45.1) and Il2rb Mut (CD45.2) chimeric mice. (E) ImageStream quantification, with membrane intensity/area, for WT (black) and Il2rb Mut (red). (F) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 6 per genotype. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (opened circles) and TEM cells (opened diamonds), n = 6 per genotype. (I) T cell distribution, n = 7 per genotype, over three experiments. Comparison of TEM cell numbers between genotypes, n = 4 per genotype (right). Data show one representative experiment. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.
    Figure Legend Snippet: Mixed (WT:WT or Mut:WT at 97:3, into WT) bone marrow chimera (BMC) mouse cells and sera were harvested and analyzed 12–14 weeks post-transplant. Black open shapes indicate WT cells from Mut:WT BMCs (WT/Mut:WT). Red open shapes indicate Il2rb Mut cells from Mut:WT BMCs (Mut/Mut:WT). Data depict one representative experiment in each of (A)–(I). (A) Serum cytokine levels of IL-2, n = 3 for WT:WT and n = 4 for Mut:WT, and of IL-15c, n = 2 for WT:WT and n = 4 for Mut:WT, measured as in . (B) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 6 per genotype; isotype control antibody MFI is indicated by the gray dotted line. (C) CD25 expression depicted as in (B), n = 6 per genotype. (D) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (CD45.1) and Il2rb Mut (CD45.2) chimeric mice. (E) ImageStream quantification, with membrane intensity/area, for WT (black) and Il2rb Mut (red). (F) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 6 per genotype. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (opened circles) and TEM cells (opened diamonds), n = 6 per genotype. (I) T cell distribution, n = 7 per genotype, over three experiments. Comparison of TEM cell numbers between genotypes, n = 4 per genotype (right). Data show one representative experiment. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Techniques Used: Expressing, Fluorescence, Control, Membrane, Staining, Activation Assay, Comparison, MANN-WHITNEY

    Analyses of splenocytes and serum from mice age 13 weeks. Genotypes include WT, n = 6 (black), and Il2rb Mut , n = 2 (red). Transferred Tregs are defined as CD4 + CD25 + GFP + . (A) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) were measured by ELISA. (B) Body weight of neonatal WT or Il2rb Mut mice with (right) and without (left) WT Treg transfer. (C) Spleen weight. (D) Representative dot plots depicting T cell subsets, CD4 + (left) and CD8 + (right). Naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for both genotypes. (E) Average T cell distribution for all populations and genotypes. (F) Total CD122 expression depicted as median fluorescence intensity (MFI) in subsets of naive (circles) and TEM (diamonds) cells; isotype control antibody MFI is indicated by the gray dotted line. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in naive (circles) and TEM (diamonds) cells. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor, displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.
    Figure Legend Snippet: Analyses of splenocytes and serum from mice age 13 weeks. Genotypes include WT, n = 6 (black), and Il2rb Mut , n = 2 (red). Transferred Tregs are defined as CD4 + CD25 + GFP + . (A) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) were measured by ELISA. (B) Body weight of neonatal WT or Il2rb Mut mice with (right) and without (left) WT Treg transfer. (C) Spleen weight. (D) Representative dot plots depicting T cell subsets, CD4 + (left) and CD8 + (right). Naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for both genotypes. (E) Average T cell distribution for all populations and genotypes. (F) Total CD122 expression depicted as median fluorescence intensity (MFI) in subsets of naive (circles) and TEM (diamonds) cells; isotype control antibody MFI is indicated by the gray dotted line. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in naive (circles) and TEM (diamonds) cells. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor, displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence, Control, Activation Assay, MANN-WHITNEY

    Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). The bivariate (right), representing each subpopulation from both genotypes across three experiments, shows unstimulated vs. maximum IL-2 and IL-15c concentrations. See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.
    Figure Legend Snippet: Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). The bivariate (right), representing each subpopulation from both genotypes across three experiments, shows unstimulated vs. maximum IL-2 and IL-15c concentrations. See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.

    Techniques Used: Control, Comparison

    Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.
    Figure Legend Snippet: Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.

    Techniques Used: Control, Comparison



    Similar Products

    il2rb mut mice young cohort t cell analysis  (Mendeley Ltd)
    86
    Mendeley Ltd il2rb mut mice young cohort t cell analysis
    Genotypes shown include WT (black) and <t>Il2rb</t> Mut (red). (A)–(E) each represent three individual experiments. (A) Total body or spleen weight of 6- to 8-week littermates, n = 15 per genotype. (B) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) comparing mice at age 4 weeks (left, WT, n = 5; Il2rb Mut , n = 6) and 7 weeks (right, IL-2, n = 19 per genotype; IL-15c, n = 17 for WT and n = 18 for Il2rb Mut ). (C) Representative dot plot depicting Tregs defined as CD4 + CD25 + Foxp3 + for genotypes indicated (left). Percentage of Tregs from CD4 + T cells, n = 11 per genotype (right). (D) Representative dot plot depicting subpopulations of CD4 + or CD8 + T cells: naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for genotypes indicated (left). Stacked graphs showing T cell distribution, n = 11 per genotype (right). (E) Absolute numbers of Tregs–CD4 + CD25 + Foxp3 + (left), n = 11 per genotype. Absolute numbers of TEM–CD44 + CD62L − cells (right), n = 7 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor; displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns, non-significant.
    Il2rb Mut Mice Young Cohort T Cell Analysis, supplied by Mendeley Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il2rb mut mice young cohort t cell analysis/product/Mendeley Ltd
    Average 86 stars, based on 1 article reviews
    il2rb mut mice young cohort t cell analysis - by Bioz Stars, 2026-06
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(E) each represent three individual experiments. (A) Total body or spleen weight of 6- to 8-week littermates, n = 15 per genotype. (B) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) comparing mice at age 4 weeks (left, WT, n = 5; Il2rb Mut , n = 6) and 7 weeks (right, IL-2, n = 19 per genotype; IL-15c, n = 17 for WT and n = 18 for Il2rb Mut ). (C) Representative dot plot depicting Tregs defined as CD4 + CD25 + Foxp3 + for genotypes indicated (left). Percentage of Tregs from CD4 + T cells, n = 11 per genotype (right). (D) Representative dot plot depicting subpopulations of CD4 + or CD8 + T cells: naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for genotypes indicated (left). Stacked graphs showing T cell distribution, n = 11 per genotype (right). (E) Absolute numbers of Tregs–CD4 + CD25 + Foxp3 + (left), n = 11 per genotype. Absolute numbers of TEM–CD44 + CD62L − cells (right), n = 7 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor; displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns, non-significant.

    Journal: Cell reports

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    doi: 10.1016/j.celrep.2025.115902

    Figure Lengend Snippet: Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(E) each represent three individual experiments. (A) Total body or spleen weight of 6- to 8-week littermates, n = 15 per genotype. (B) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) comparing mice at age 4 weeks (left, WT, n = 5; Il2rb Mut , n = 6) and 7 weeks (right, IL-2, n = 19 per genotype; IL-15c, n = 17 for WT and n = 18 for Il2rb Mut ). (C) Representative dot plot depicting Tregs defined as CD4 + CD25 + Foxp3 + for genotypes indicated (left). Percentage of Tregs from CD4 + T cells, n = 11 per genotype (right). (D) Representative dot plot depicting subpopulations of CD4 + or CD8 + T cells: naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for genotypes indicated (left). Stacked graphs showing T cell distribution, n = 11 per genotype (right). (E) Absolute numbers of Tregs–CD4 + CD25 + Foxp3 + (left), n = 11 per genotype. Absolute numbers of TEM–CD44 + CD62L − cells (right), n = 7 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor; displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns, non-significant.

    Article Snippet: Il2rb Mut mice young cohort T Cell Analysis , Mendeley Data , https://doi.org/10.17632/tkwmmd27jh.1.

    Techniques: MANN-WHITNEY

    Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(H) each represent 2 individual experiments. (A) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT, n = 14 for Il2rb Mut ; isotype control antibody MFI is indicated by the gray dotted line. (B) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (top) and Il2rb Mut (bottom). (C) ImageStream quantification, with membrane intensity/area. (D) CD25 expression depicted as in (A), n = 8 for WT and n = 9 for Il2rb Mut . (E) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 7 per genotype. (F and G) Activation and exhaustion markers PD-1 (F) and KLRG1 (G) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT and n = 14 for Il2rb Mut . (H) Representative dot plot distinguishing BM subsets, specifically LSK (Sca-1 + c-Kit + ) cells (top right quad) and myeloid progenitors (MPs; top left quad). Graphs show percentage of LSK cells (left) and MP cells (right), n = 6 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Journal: Cell reports

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    doi: 10.1016/j.celrep.2025.115902

    Figure Lengend Snippet: Genotypes shown include WT (black) and Il2rb Mut (red). (A)–(H) each represent 2 individual experiments. (A) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT, n = 14 for Il2rb Mut ; isotype control antibody MFI is indicated by the gray dotted line. (B) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (top) and Il2rb Mut (bottom). (C) ImageStream quantification, with membrane intensity/area. (D) CD25 expression depicted as in (A), n = 8 for WT and n = 9 for Il2rb Mut . (E) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 7 per genotype. (F and G) Activation and exhaustion markers PD-1 (F) and KLRG1 (G) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (circles) and TEM cells (diamonds), n = 12 for WT and n = 14 for Il2rb Mut . (H) Representative dot plot distinguishing BM subsets, specifically LSK (Sca-1 + c-Kit + ) cells (top right quad) and myeloid progenitors (MPs; top left quad). Graphs show percentage of LSK cells (left) and MP cells (right), n = 6 per genotype. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Article Snippet: Il2rb Mut mice young cohort T Cell Analysis , Mendeley Data , https://doi.org/10.17632/tkwmmd27jh.1.

    Techniques: Expressing, Fluorescence, Control, Membrane, Staining, Activation Assay, MANN-WHITNEY

    Mixed (WT:WT or Mut:WT at 97:3, into WT) bone marrow chimera (BMC) mouse cells and sera were harvested and analyzed 12–14 weeks post-transplant. Black open shapes indicate WT cells from Mut:WT BMCs (WT/Mut:WT). Red open shapes indicate Il2rb Mut cells from Mut:WT BMCs (Mut/Mut:WT). Data depict one representative experiment in each of (A)–(I). (A) Serum cytokine levels of IL-2, n = 3 for WT:WT and n = 4 for Mut:WT, and of IL-15c, n = 2 for WT:WT and n = 4 for Mut:WT, measured as in . (B) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 6 per genotype; isotype control antibody MFI is indicated by the gray dotted line. (C) CD25 expression depicted as in (B), n = 6 per genotype. (D) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (CD45.1) and Il2rb Mut (CD45.2) chimeric mice. (E) ImageStream quantification, with membrane intensity/area, for WT (black) and Il2rb Mut (red). (F) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 6 per genotype. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (opened circles) and TEM cells (opened diamonds), n = 6 per genotype. (I) T cell distribution, n = 7 per genotype, over three experiments. Comparison of TEM cell numbers between genotypes, n = 4 per genotype (right). Data show one representative experiment. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Journal: Cell reports

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    doi: 10.1016/j.celrep.2025.115902

    Figure Lengend Snippet: Mixed (WT:WT or Mut:WT at 97:3, into WT) bone marrow chimera (BMC) mouse cells and sera were harvested and analyzed 12–14 weeks post-transplant. Black open shapes indicate WT cells from Mut:WT BMCs (WT/Mut:WT). Red open shapes indicate Il2rb Mut cells from Mut:WT BMCs (Mut/Mut:WT). Data depict one representative experiment in each of (A)–(I). (A) Serum cytokine levels of IL-2, n = 3 for WT:WT and n = 4 for Mut:WT, and of IL-15c, n = 2 for WT:WT and n = 4 for Mut:WT, measured as in . (B) Total CD122 expression depicted as median fluorescence intensity (MFI) in subpopulations of splenic CD4 + and CD8 + T from both genotypes; naive (circles) and TEM cells (diamonds), n = 6 per genotype; isotype control antibody MFI is indicated by the gray dotted line. (C) CD25 expression depicted as in (B), n = 6 per genotype. (D) CD122 cell-surface expression (purple) measured by ImageStream. Membrane mask was applied to the analysis of cells stained with CD122 clone 5H4 (cell surface + intracellular). Images compare CD4 + (green) to CD8 + (yellow) splenocytes from WT (CD45.1) and Il2rb Mut (CD45.2) chimeric mice. (E) ImageStream quantification, with membrane intensity/area, for WT (black) and Il2rb Mut (red). (F) Frequency of IL-2-, IFN-γ-, granzyme B-, and perforin-producing CD4 + and CD8 + splenic T cells, n = 6 per genotype. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in CD4 + and CD8 + splenocytes from both genotypes; naive (opened circles) and TEM cells (opened diamonds), n = 6 per genotype. (I) T cell distribution, n = 7 per genotype, over three experiments. Comparison of TEM cell numbers between genotypes, n = 4 per genotype (right). Data show one representative experiment. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor. ImageStream statistics calculated by unpaired parametric Welch’s correction are displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Article Snippet: Il2rb Mut mice young cohort T Cell Analysis , Mendeley Data , https://doi.org/10.17632/tkwmmd27jh.1.

    Techniques: Expressing, Fluorescence, Control, Membrane, Staining, Activation Assay, Comparison, MANN-WHITNEY

    Analyses of splenocytes and serum from mice age 13 weeks. Genotypes include WT, n = 6 (black), and Il2rb Mut , n = 2 (red). Transferred Tregs are defined as CD4 + CD25 + GFP + . (A) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) were measured by ELISA. (B) Body weight of neonatal WT or Il2rb Mut mice with (right) and without (left) WT Treg transfer. (C) Spleen weight. (D) Representative dot plots depicting T cell subsets, CD4 + (left) and CD8 + (right). Naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for both genotypes. (E) Average T cell distribution for all populations and genotypes. (F) Total CD122 expression depicted as median fluorescence intensity (MFI) in subsets of naive (circles) and TEM (diamonds) cells; isotype control antibody MFI is indicated by the gray dotted line. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in naive (circles) and TEM (diamonds) cells. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor, displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Journal: Cell reports

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    doi: 10.1016/j.celrep.2025.115902

    Figure Lengend Snippet: Analyses of splenocytes and serum from mice age 13 weeks. Genotypes include WT, n = 6 (black), and Il2rb Mut , n = 2 (red). Transferred Tregs are defined as CD4 + CD25 + GFP + . (A) Serum cytokine levels of IL-2 and IL-15c (IL-15/15Rα) were measured by ELISA. (B) Body weight of neonatal WT or Il2rb Mut mice with (right) and without (left) WT Treg transfer. (C) Spleen weight. (D) Representative dot plots depicting T cell subsets, CD4 + (left) and CD8 + (right). Naive, lower right quad; central memory (TCM), upper right quad; and effector memory (TEM), upper left quad, for both genotypes. (E) Average T cell distribution for all populations and genotypes. (F) Total CD122 expression depicted as median fluorescence intensity (MFI) in subsets of naive (circles) and TEM (diamonds) cells; isotype control antibody MFI is indicated by the gray dotted line. (G and H) Activation and exhaustion markers PD-1 (G) and KLRG1 (H) measured in naive (circles) and TEM (diamonds) cells. Data are shown as mean ± SEM. All p values were calculated by non-parametric Mann-Whitney test for a single factor, displayed as **** p ≤ 0.0001, *** p ≤ 0.001, ** p ≤ 0.01, and * p ≤ 0.05 or ns.

    Article Snippet: Il2rb Mut mice young cohort T Cell Analysis , Mendeley Data , https://doi.org/10.17632/tkwmmd27jh.1.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence, Control, Activation Assay, MANN-WHITNEY

    Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). The bivariate (right), representing each subpopulation from both genotypes across three experiments, shows unstimulated vs. maximum IL-2 and IL-15c concentrations. See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.

    Journal: Cell reports

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    doi: 10.1016/j.celrep.2025.115902

    Figure Lengend Snippet: Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). The bivariate (right), representing each subpopulation from both genotypes across three experiments, shows unstimulated vs. maximum IL-2 and IL-15c concentrations. See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.

    Article Snippet: Il2rb Mut mice young cohort T Cell Analysis , Mendeley Data , https://doi.org/10.17632/tkwmmd27jh.1.

    Techniques: Control, Comparison

    Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.

    Journal: Cell reports

    Article Title: A hypomorphic Il2rb mutant mouse model recapitulates and reveals mechanisms of human T cell immune dysregulation in IL-2Rβ deficiency

    doi: 10.1016/j.celrep.2025.115902

    Figure Lengend Snippet: Splenocytes were stimulated with IL-7 and increasing doses of IL-2 (top) and IL-15 (bottom). Genotypes shown include WT (black) and Il2rb Mut (red). Transferred Tregs are marked by CD4 + CD25 + GFP + . Bar graphs (left) quantify pSTAT5 response as a percentage of CD8 + T naive and TEM cells, based on the threshold set on unstimulated condition and isotype control for pSTAT5 antibody MFI per representative animal per genotype. (A) Intact animals, n = 3 per genotype. (B) Mut:WT-mixed BMCs, n = 6, with congenic WT (black) and Il2rb Mut (red) donors. (C) Foxp3 − GFP + Tregs transferred into neonatal WT ( n = 6) and Il2rb Mut ( n = 2). See for p value calculations. The p values are indicated by letters (a)–(h) for each comparison performed per cytokine.

    Article Snippet: Il2rb Mut mice young cohort T Cell Analysis , Mendeley Data , https://doi.org/10.17632/tkwmmd27jh.1.

    Techniques: Control, Comparison